Vol. 10, Issue 12 (2021)

Author(s):
Sanchit Thakur, Jai Dev Sharma and Kamal Dev Sharma

Abstract:
Confirmation of hybridity is essential to exclude selfed plants in plant breeding programmes. The morphological markers for confirmation of hybridity in chickpea are unavailable in closely related germplasm and this results in ambiguity in identification of true hybrids. In present study, cold tolerant parent ICC-16349 (donor) and cold susceptible parent GPF-2 (recipient) were crossed to generate 80 putative hybrids. The parents were screened using 51 simple sequence repeat (SSR) markers, of which only one i.e TA 180 exhibited polymorphism. Screening of 80 putative hybrids using TA 180 revealed that only 34 (42.5%) of the putative hybrids were true hybrids. The study indicated that in chickpea breeding programmes, hybridity must be confirmed using DNA based markers to avoid inclusion of selfed plants as hybrids.