Vol. 11, Issue 3 (2022)

Author(s):
VC Khelurkar, MP Moharil, SB Sakhare, PV Jadhav, NR Potdukhe, AW Thorat, PP Thakur, SS Mane and RB Ghorade

Abstract:
Simple sequence repeat markers were used for fingerprinting of hybrids, assessing variation within parental lines, and testing the genetic purity of hybrid seed lot in chickpea as a self-pollinating crop. In this study, 10 simple sequence repeats (SSR) markers were employed for parental polymorphism and F₁ hybridity assessments. One TA-14 SSR marker was found polymorphic between the used parents and produced a specific allele for each parent. This TA-14 SSR marker differentiated the 45 F1 hybrids from 70 F₁’s plants for their true hybrid nature. This marker can be used as referral markers for the unambiguous identification and protection of these hybrids. The analysis of plant-to-plant variation within the parental lines of the hybrid, using informative markers indicated residual heterozygosity at marker loci. This highlights the importance of SSR markers in maintaining the genetic purity of the parental lines. To utilize this SSR marker effectively for the detection of impurities in hybrids. Hence, The SSR marker information developed through this study will be of immense help for the hybrid seed industry to select the genetic purity of the crop.