Vol. 11, Issue 3 (2022)

Author(s):
Jisna George, Gitanjali Devi and Badal Bhattacharyya

Abstract:
Entomopathogenic nematode, Heterorhabditis bacteriophora was formulated in six different formulations such as talc, sawdust, alginate gel, water dispersible granule and compost: charcoal powder mixture. All formulations tested in vitro at two temperatures (5 ^°C and 30 ^°C) to evaluate their survival and infectivity against wax moth, Galleria mellonella. Alginate gel and Sawdust formulations enhanced highest survival of infective juveniles of H. bacteriophora. Mean percent survival of H. bacteriophora infective juveniles (IJs) storage at 5 ^°C in alginate gel was 99.40% and in sawdust 98.76%, whereas mean percent survival was less (98.90% and 98.36% respectively) at 30 ^°C. Mean larval mortality of G. mellonella by H. bacteriophora stored at 5 ^°C in alginate gel was 66.66% and 63.33% in sawdust formulation whereas larval mortality stored at 30 ^°C in alginate gel was 59.33% and 56.00% in sawdust formulation. However from economic point of view sawdust formulation seems to be more efficient formulation.