Vol. 10, Issue 9 (2021)

Author(s):
Rohit Kambale, Veera Ranjani Rajagopalan, Bharathi Ayyenar and Raveendran Muthurajan

Abstract:
Rice (Oryza sativa L.) is a major staple food crop grown over hundred different countries. To feed the ever-increasing population by 2050, global food production must be doubled. Rice productivity has been stagnant due to a number of biotic and abiotic factors. Abiotic stress, on the other hand, create significant economic losses by lowering yield and quality. The quality of cereals is negatively affected by heat stress and cause loss of >10% of grain production. This warrants empowering rice crop to withstand ill effects of heat. It requires discovery of genetic factors regulating heat tolerance traits and discovery of genes and their elite alleles associated with heat tolerance traits. The current study attempted to survey the allelic variation in a putative heat tolerant gene OsMADS87 (LOC Os03g38610) using insilico analysis. OsMADS87 (LOC Os03g38610), a MADS box transcription factor influencing seed size and thermo sensitivity, contains one exon with a transcript length of 750 bps. Its allelic variation was surveyed across a set of 201 aus rice accessions from 3K rice genome project. Allelic diversity analysis identified 10 SNPs and 11 INDEL positions in OsMADS87 gene sequence. Ten haplotypes (H1, H2, H3 and H4) contributed by 10 non synonymous SNPs were identified.