Vol. 11, Issue 3 (2022)

Author(s):
Kalyani More, AA Bharose, Balaji Jadhav and SM Umate

Abstract:
Two types of molecular markers, random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR), were assayed to determine the genetic diversity of 10 wheat cultivars. A high level of polymorphism was found with both RAPD and ISSR markers. In RAPD analyses, 200 out of260 bands (73.71%) were polymorphic. The RAPD primer S133 and S138 showed 100% polymorphism while S32 primer showed only 31.03% polymorphism. The cluster analysis reveals that the two clusters contain two major three minor clusters and one out group. In SSR analyses, a total of 118 alleles were detected, among which 68 alleles (56.88%) were polymorphic. PIC value of 0.97 was WMC-156 while the least informative marker was found to be GWM-140 with PIC value of 0.15 amplified alleles ranged from 1 to 3 in all the wheat genotypes. The cluster analysis reveals that the two clusters contain two major three minor clusters and four out group.