Vol. 11, Issue 4 (2022)

Author(s):
Velprabakaran Senthilvel, Vanniarajan Chockalingam, Renuka Raman, Shanmugasundaram Rangasamy, Thiruvudainambi Sundararajan and Ramalingam Jegadeesan

Abstract:
Bacterial blight (BB) and phosphorus starvation tolerance are key factors that limit the rice production. Resistant cultivars developments are an excellent approach for managing biotic and abiotic stress challenges at a low cost. Morphological and genotypic screenings are commonly used method in the marker assisted crop improvement programme. Improved recombinant lines were screened by using functional markers and resistance reaction was confirmed through phenotypic screening. An active virulent local strain of Xanthomonas oryzae Pv. Oryaze (Xoo), IS (PNT-4) was employed to identify the resistant genotypes from the homozygous lines developed from popular rice varieties (ADT43, ASD16 and Co51), and donor lines (IRBB60, ACM18220, ISM pup1) combinations. Pyramided Pup1 lines were phenotypically evaluated under hydroponic condition. The improved lines were confirmed by using functional markers PTA248, xa13, xa5, K29-3 and K46-1 to identify BB (Xa21, xa13, xa5,) and Phosphorus starvation tolerance (OsPSTOL1) genes. Among the genotypically confirmed twenty seven RILs, (AD (Bio) 13056, AD(Bio)13060, ACM18089,ACM18091, ACM18097, ACM18068, and ACM20015 were found to be promising for agronomic traits. These pyramided lines will be useful to breeders to develop newer variety/donor line in stress breeding programme.