Vol. 11, Issue 4 (2022)

Author(s):
Kalaivani K, Rajadurai KR, Hemaprabha K and Kannan M

Abstract:
Investigation on “In vitro propagation of Dracaena sanderiana cv. Victory through direct organogenesis” was carried out at the Tissue Culture laboratory of the Horticultural College and Research Institute of TNAU, Coimbatore. In Dracaena sanderiana cv. Victory, shoot tips and nodal segments were used as explants. Of these, shoot tips were found to be the most suitable explants in response to in vitro regeneration and multiplication. The results revealed that the highest survival percentage was recorded by 0.1% Bavistin (30 min.) + 1.5% NaOCl (10 min.) + 70% Ethanol (2 min.) + 0.1% HgCl₂ (10 min.) in Shoot tips and by 0.1% Bavistin (30 min.) + 1.5% NaOCl (15 min.) + 70% Ethanol (2 min.) + 0.1% HgCl₂ (15 min.) in nodal segments with minimum per cent of contamination and mortality. The results revealed that MS medium + BAP (3 mg l^-1) + NAA (0.1 mg l^-1) was found to be most conducive for the explants with respect to days required for shoot emergence, maximum percentage response to shoot proliferation, number of shoots per explant and average length of shoots.