Vol. 11, Issue 4 (2022)

Author(s):
K Antony Prajwala, BVK Bhagavan, K Ravindra Kumar, P Subbaramamma, K Prabhakara Rao and T Gouri Sankar

Abstract:
An investigation was undertaken to standardise the in vitro culture establishment in pomegranate cultivars Bhagwa and Super Bhagwa by using different combinations and concentrations of growth regulators. Among different treatments tested, it was observed that The explants of pomegranate cv. Bhagwa inoculated on MS media supplemented with different combinations and concentrations of plant growth regulators had performed well over the explants cv. Super Bhagwa in terms of number of sprouts explant^-1 (2.34), shoot length (2.41cm), percent callus induction (1.81%) and culture establishment index (140.42). The culturing of nodal explants of pomegranate cultivars on MS media supplemented with 1mgL^-1 of BAP and 0.5 mgL^-1 of NAA (T₂) had recorded the less number of days for bud initiation (10.45d), maximum percent (86.83%) of culture establishment, number of sprouts (4.49), leaves (7.83), shoot length (4.05cm) explant^-1 and culture establishment index (390.52) over other treatments and the control treatment showed no culture establishment.