Vol. 11, Issue 5 (2022)

Purpose: The study on biochemical and hematological studies of Pati duck (Anas platyrhynchos domesticus) of Assam at their different stages of development is of great value in regard to diseases control. The aim of the study was to evaluate the biochemical and hematological studies of Pati duck of Assam at their different stages of development.

Materials and Methods: The present studies were conducted on 45 numbers of Pati duck of Assam of irrespective of sex at different stages of development. The birds were divided into five groups viz., 1st week, 4^th week, 16^th week, 24^th week and 42^nd week of age. The ducks were procured from Pathsala and nearby area of Bajali district of Assam. 10ml of blood samples were collected from each bird of each experimental group, 5ml of which were transferred to sterilized test tube containing EDTA for hematological study viz. Total Leucocytes Count, Total Erythrocytes Count, Haemoglobin and Packed Cell Volume. The remaining 5 ml blood were transferred to 15ml glass centrifuged tube to be centrifuged at 3000 rpm for 15 minutes, the supernatant (serum) of which were utilized for different biochemical test viz. alkaline phosphatase and acid phosphatase. The data were analyzed as per methods described by Snedecor and Cochran (1994) and were presented accordingly.

Results: In the present study the serum alkaline phosphatase was found in Pati ducks at 1^st, 4^th, 16^th, 24^th and 42^nd week of age was 337.01 ± 8.77 U/L, 194.46 ± 1.28 U/L, 123.02 ± 4.98 U/L, 103.79 ± 0.97 U/L and 72.33 ± 3.07 U/L, respectively. The serum acid phosphatase level estimated in the present study was found in Pati duck recorded as was 5.11 ± 2.12 IU/L,6.09 ± 0.81 IU/L,7.90 ± 1.76 IU/L, 5.35 ± 0.23 IU/L and 7.27 ± 0.59 IU/L during 1^st, 4^th, 16^th, 24^th and 42^nd week of age of duck, respectively. The mean value of hemoglobin in 1^st week, 4^th week, 16^th week, 24^th week and 42^th week old duck were 12.29 ± 0.64 g/dl, 14.93 ± 1.42 g/dl, 17.26 ± 0.30 g/dl, 16.01 ± 1.00 g/dl and 15.38 ± 0.42 g/dl, respectively. The average Total Leucocytes Count levels in 1^st and 16^th week age groups were found to be 55.78± 4.16 m/mm³ and 66.52 ± 0.32 m/mm³, respectively. There was significant difference (P< 0.05) of Total Leucocytes Count level between age group I and III and also between group II and IV. The average Total Erythrocyte Count level in group I. II, III, IV and V were 2.28 ± 0.15 m/mm³, 2.07 ± 0.08 m/mm³, 2.05 ± 0.01 m/mm³, 2.30 ± 0.09 m/mm³ and 2.44 ± 0.03 m/mm³, respectively. The average value of Packed Cell Volume was 36.52 ± 1.09,40.10 ± 1.69, 34.81 ± 0.53,41.21± 0.82 and 42.38 ± 0.40% during 1^st week, 4^th week, 16^th week, 24^th week and 42 weeks of age of Pati duck, respectively.