Vol. 11, Issue 5 (2022)

Author(s):
Khalid Hussain Salaria, Sanjay Guleria, Aasiya Majeed, Pooja Choudhary and Faiqa Aiman

Abstract:
In the present study, probiotic bacteria L. plantarum (MTCC-1407)was used for augmenting polyphenol content, antioxidant and anti-tyrosinase activity of O. sanctum methanolic extract. Optimization of fermentation parameters namely, inoculum load, pH, temperature and incubation time was done. DPPH radical scavenging activity and reducing power assays were performed for determining the antioxidant activity. Tyrosinase inhibitory activity assay was carried out to evaluate anti-melanogenesis property of plant extract. Results indicated that the optimized fermentation parameters viz., inoculum load was 3.55 Log CFU/mL, pH was 6.5, temperature was 37 ºC and incubation time was 42 h. TPC was increased by 16.66 percent, TFC was enhanced by 24.25 percent, DPPH radical scavenging activity was increased by 4.54 percent reducing power was augmented by 4.00 percent and tyrosinase inhibitory activity was increased by 40.20 percent. These results provide the foundation for further exploration of the therapeutic and functional benefits of L. plantarum (MTCC-1407)mediated fermentation of O. sanctum methanolic extract. Furthermore, fermentation based biotransformation can be used as an alternative natural mechanisms for improvement of phytochemical constituents and bioactive properties of methanolic extract of O. sanctum.