Vol. 11, Issue 5 (2022)

Author(s):
Shipra Jaiswal, Sarita Arya and Tarun Kant

Abstract:
Pterocarpus marsupium belongs to family Fabaceae and its role as anti-diabetic has been very well established. Pterocarpus marsupium extract also shows promising results in cataract and hypertriglyceridaemia. This plant also finds its use as cardiotonic and hepatoprotective agent. The effective protocol of in vitro callus induction would aid secondary metabolite and its commercial production. In the present study, callus culture was raised for Pterocarpus marsupium from In vitro raised leaves and cotyledonary explants which were taken from seedlings germinated on MS supplemented with auxin. The objective of the research is to induction of callus and maintains the callus culture. Results illustrated that leaves obtained from in vitro raised shoots were not responsive in any treatment and only the cotyledons showed callus induction. Callus induction was maximum 62.22% at 9.04µM 2,4- D.