Vol. 11, Issue 5 (2022)

Author(s):
Rajasekhar P, Arunkumar H, Prasanna SB and Prabha R

Abstract:
A total of 14 oleaginous isolates obtained from dairy environmental samples like dung, feed, fodder, sewage, silage and soil samples were subjected for secondary screening of cells for Sudan black. Once intracellular lipid accumulation was confirmed, through smear, the isolates were phenotyped by subjecting to preliminary and specific tests and based on which the isolates belonged to species of Candida (6), Kluveromyces (3), Rhodotrula (1), Saccharomyces (2) and Yarrowia (2). Species of Candida predominated among the phenotype accounting for 42.86% followed by 21.43% of Kluveromyces, 14.29 each of Saccharomyces and Yarrowia and finally 7.14% of Rhodotorula. pH of yeast cultured MGYP broth showed values in the range of pH 4.6 to 4.3 from initial pH of 6.4 with DMC of 8.00 to 8.80 log₁₀/ml. Soil yeast isolates Y10, phenotyped as Yarrowia sp. and Y14 as Candida sp. had DMC of 8.80 and 8.60 log₁₀/ml respectively. Dry cell mass of 14 yeast isolates ranged from 10.30 to 57.00 g/l and yield of lipid was 2.80 to 29.00 g/l with per cent lipid of 17.86 to 66.25 per cent. The highest producer of lipid was Y10, Yarrowia sp., with 66.25 per cent while Y14, Candida sp., had 58.18 per cent of lipid.