Vol. 11, Issue 5 (2022)

Author(s):
Srishti Soni, Reena Mukherjee, Ujjwal Kumar De, Deeksha Bharti, Mamta Singh, Bhagirathi, Babul Rudra Paul, Jitendra Singh Gandhar, Varun Kumar Sarkar, Amit Kumar, R Raghuvaran and Ravi Kumar Soni

Abstract:
Antibiotic resistance is an emerging problem faced by the dairy industry. To improve the targeted drug delivery and bioavailibilty, nanotechnology is such an alternative and fascinating developing field of science. In this study Baicalein, traditional Chinese medicine due to its the intrinsic poor solubility and low bioavailability limits its biomedical applications. Chitosan encapsulation was performed with resistant beta-lactam antibiotic, amoxicillin against Methicillin resistant Staphylococcus aureus. This study envisages the preparation of Chitosan encapsulated baicalein and amoxicillin for increasing bioavailability and enhancing targeted delivery. The yield of the Chitosan encapsulated baicalein and amoxicillin particles was 83.4±3.04959% and the corresponding encapsulation efficiency was 83.8 ±2.58%. The size distribution of the chitosan encapsulated amoxicillin and baicalein particles were of size 251nm. The in vitro release study revealed a 28.3 percent burst release in the initial 6 hours, followed by a sustained slow and steady release of baicalein from the chitosan encapsules. This is important to maintain the minimum drug concentration at the site of infection. and improve the therapeutic amoxicillin release profile showed significant release of 32% in the first 5 hours, followed by extended release. These findings imply that CH encapsulated BC with beta-lactam could be a promising mastitis treatment.