Vol. 11, Issue 6 (2022)

Author(s):
Madhuri Pradhan and Debendranath Bastia

Abstract:
The present investigation was carried out during Rabi season, 2017 at the Rice Research Station and Sinha molecular laboratory, College of Agriculture, Odisha University of Agriculture and Technology, Bhubaneswar. Two rice varieties i.e., Pratikshya and Swarna MAS, were screened for bacterial leaf blight genes. To examine the presence of any blight resistance genes, phenotypic screening was done by using leaf clip inoculation method with isolates of Xanthomonas oryzae pv. oryzae (Xoo) for blight in field and genotyping for three major blight (xa5, xa13 and Xa21) resistant genes was carried out by using gene linked markers RM164, RM230 and RM21 respectively. Both phenotypic screening and genotyping results showed that Pratikshya was highly susceptible, while Swarna MAS was resistant to bacterial leaf blight. This experiment is helpful for designing improvement of bacterial leaf blight resistant versions of rice by gene pyramiding with help of molecular markers.