Vol. 11, Issue 6 (2022)

Author(s):
Shital M Padhiyar, Jasminkumar Kheni, Hiral V Desai Rukam Singh Tomar and HP Gajera

Abstract:
The nutritional value of millet is dependent upon its metabolic profile, including the types and amounts of natural phenolic compounds present, which warrant evaluation in order to determine the best genotypes for human consumption. Barnyard millet (Echinochloa frumentacea L.) could be a good source of iron for vegetarians. In the present study, Metabolome profiling of barnyard millet genotypes containing high Fe and low Fe content in five spike development stage was performed using GC-MS platform and results observed highest percentage of sugar and sugar alcohol (34%) followed by organic acid (26%), Amino acids, sterol, other compound (23%) and fatty acid (17%) in spike development stages of Low Fe Vs High Fe genotypes. Heat map revealed that during spike emergence the metabolic compounds like d-Ribose, D-Fructose, D-Glucose, Galactose, D-Turanose, Glucopyranose, D-Mannitol, Hexadecanoic acid, Docosanoic acid, alpha. - Glycerophosphoric acid and beta.-Sitosterol were found high in high Fe genotype in comparison to Low Fe genotype. The hierarchical cluster analysis, revealed that high Fe five spike development stages shares close metabolite pool to each other and in low Fe four spike development stages also shares similarly metabolite pool to each other except spike emergence stage of Low Fe genotype. PCA showed that the expression patterns of the five developmental stages differed significantly.