Vol. 11, Issue 6 (2022)

Author(s):
Kamlesh Ram

Abstract:
Lentil is one of the major rabi pulse crop grown in India. It is a rich source of protein, minerals and vitamins for human nutrition and straw is also valued animal feed. The maximum radial growth (86.33mm) at seven days with maximum mycelial dry weight (326.11mg) at twenty-one days after inoculation at 25±2 ℃ was observed. The next best culture media were oat meal agar and Richard’s agar which showed 80.33mm and 75.66mm radial growth and 295.20mg and 288.10mg of dry weight. Lentil meal agar was the least effective, showing 37.00mm of radial growth and 186.90mg of mycelial dry weight. This fungus sporulates in all test media, but excellent sporulation was recorded in potato dextrose agar and Richard’s agar media. The maximum radial growth at pH 6.0 (88.66mm) and pH 6.5 (84.33mm) were also found to be favorable. The highly alkaline and acidic pH is not favourable for the best growth and sporulation of Fusarium oxysporum f. sp. lentis. Maximum radial growth was also found to be favourable 84.00mm at 30 ℃ and next best 78.66mm at 25±2 ℃. The growth and sporulation of the test pathogen is slow when the temperature is increased and decreased at 30 °C.