Vol. 11, Issue 6 (2022)

Author(s):
Balaji C Jadhav, Mahendra S Dudhare, Kalyani More, Hanuman A Malge, Devendra Payasi and MK Ghodke

Abstract:
Two types of molecular markers, Inter Simple Sequence Repeats (ISSR) and simple sequence repeat (SSR), were used to determine the genetic diversity and molecular characterisation of 21 linseed lines. The present SSR primers investigation revealed that genotypes BAU 14-03 and Sharda (ch) (72%) were found to be the most diverse linseed lines and primer LU-10 (0.79) and LU-9 (0.78) showed highest PIC value and show the 100% polymorphism. The cluster analysis based on SSR markers across various linkage groups data revealed a relatively broad genetic background of the indigenous linseed lines. Two distinct and major clusters (I and II) were found to represent unique grouping of 21 linseed genotypes. The number of genotypes in each cluster varied from 5 to 16 and ISSR investigation revealed that genotypes SLS 127 and SLS 128 (25%) were found to be the most diverse and primer UBC-825(0.29) and UBC-815(0.24) showed highest PIC value and show the 100% polymorphism. The cluster analysis based on ISSR markers across various linkage groups data revealed four distinct and major clusters (I, II, III and IV) were found to represent unique grouping of 21 linseed genotypes. The number of genotypes in each cluster varied from 2 to 10.These results indicated good sources of genetic analysis using ISSR and SSR primers which will help breeders to evaluate genetic diversity and genetic relationship among different flax genotypes.