Vol. 11, Issue 6 (2022)

Author(s):
SK Rai and Lekhraj Jat

Abstract:
Ten SSR primer were employed to generate polymorphism of 20 Brassica juncea genotypes. Primers with high polymorphic bands SSR Na14-G06 and SSR Na14-D09 were more efficient to discriminate the varieties. These primers are useful for fingerprinting in Brassica juncea lines as they were able to resolve all the genotypes. Polymorphism information content of each primer was calculated and ranged from 0.33 (primer SSR Na12_H09) to 0.79 (primer A01_268890) with an average of 0.58. Percentage of polymorphism ranged from 10.52% to 44.44% with an average of 19.85% per primer.