Abstract:An investigation was carried out during 2015-2018 to study the efficacy of green synthesized copper nanoparticles in managing leaf spot disease of chrysanthemum (
Dendranthema grandiflora) cv. Snowball. The fungal infection was studied in chrysanthemum in pot experiment and the synthesized CuNPs were targeted to the fungus in lab condition. Further, the CuNPs were also tested in vase solution of cut chrysanthemum.
A novel fungus was isolated from infected leaf of chrysanthemum (Dendranthema grandiflora) and was cultured in PDA media. The isolate was identified as Corynespora dendranthema that is reported for the first time as foliage pathogen of chrysanthemum. The efficacy of green synthesized CuNPs against the fungus was evaluated using Agar well technique. The result showed that the green synthesized CuNPs exhibited mycelial growth inhibition at 60.52%, 57.13% and 52.44% for Night jasmine, Allamanda and Yellow oleander CuNPs respectively against the mycelial growth inhibition by traditional of fungicide (48.57%). The CuNPs treatments significantly enhanced the vase life of cut chrysanthemum. Night jasmine mediated CuNPs and Allamanda mediated CuNPs at 75 ppm found to be best among all other treatments that increase vase life of cut chrysanthemum than traditional practice.
From the study inference can be drawn that green synthesis of nanoparticles from ornamental plants viz. Night jasmine, Yellow oleander and Allamanda may be superior method for management of leaf spot disease in chrysanthemum and enhancing vase life of cut chrysanthemum. However, Night jasmine mediated CuNPs was found to be more effective.