Vol. 11, Issue 7 (2022)

Author(s):
Niranjan Sannatammappa Konchigeri, Meena S, Rajesh S, Karthikeyan M and Gnanam R

Abstract:
Garlic is a one of the nature’s versatile medicinal plant, having importance in culinary purpose. It is vegetatively propagated through cloves but due to its impotency to produce potent seeds and more infection with complex of viruses leads to yield loss. Quality seed material is very important for good production, farmers are facing huge loss due to scantiness in supply of good quality seed material. The current study was performed to optimize the media for direct shoot organogenesis in garlic genotype Kodaikanal hill garlic. Micropropagation of garlic through direct organogenesis aims to produce virus free planting material. Garlic clove basal meristematic regions were studied for their in vitro responses in shoot organogenesis. The number of shoots produced through basal meristematic region explant in different shoot induction media was analyzed. MS media supplemented with 2 mg L^-1 BAP and 0.1 mg L^-1 NAA produced an average 3.1 shoots per clove. These findings of the study will be useful for scaling up the protocol for shoot organogenesis and subsequent bulblet induction in garlic.