Vol. 11, Issue 7 (2022)

Author(s):
Kirti Singh, Alok Kumar Singh, DK Dwivedi, NA Khan and SP Singh

Abstract:
The aim of this study was to identify the effect of different percentages of sodium azide mutagen on the seed germination, plumule and radicle growth so that most effective sodium azide dose can be applied for successful mutagenesis research study like TILLING. For this purpose seed of Kalanamak rice cultivar were treated with 0.01%, 0.02%, 0.03%, 0.04%, 0.05% sodium azide doses. Seed germination, embryonic root length, seedling length, seedling growth rate, fresh and dry weights of root and shoot, and root to shoot ratio were evaluated in comparison to the untreated materials. Mostly, there were statistically significant differences among sodium azide treatments for all the evaluated characters. The levels of differences were more pronounced with the increased doses of sodium azide. Thus, the selected characters evaluated in this study are relevant for the assessment of effective and optimum sodium azide treatments. In the present study, results show that a higher dose of sodium azide (0.05%) significantly reduced all the recorded parameters. The maximum value of germination (96.6%). Higher redical length (26.0mm), plumule lenghth (51.67 mm), fresh weight of plumule (39.6 g), plumule dry weight (8.6 g), radical fresh weight (14.7 g) and dry weight of radicle (4.4) were recorded in control and minimum value of these parameters at higher dose of sodium azide (0.05%).