Vol. 11, Issue 7 (2022)

Author(s):
Anklesh Katara, Mitesh Gaur, Rohit Juneja and Ishwar mal Harizan

Abstract:
The present study evaluated the beneficial effects of incorporation of caffeine as an additive in Tris fructose egg yolk citrate extender in Surti buffalo bull semen. Extended semen was divided in 4 equal parts and caffeine was added at 0.5 mM (Treatment-1), 1.0 mM (Treatment-2), 1.5 mM (Treatment-3) with one control group having no additives at all. Pre-freeze semen parameter individual progressive motility were found to be significantly higher (P< 0.05) in semen samples treated with caffeine (Treatment-1 and Treatment-2) in comparison to control. Similarly, effects of caffeine as an additive in semen were also recorded in semen thawed after 0hr and 48hr of cryopreservation. The post thaw (at 0hr) individual sperm progressive motility percentage were significantly higher (P< 0.05) in Treatment-1 and Treatment-2 compared to control. At 48hr also post thaw individual progressive motility were significantly lower (P< 0.05) in 0.5 mM caffeine (Treatment-1) and 1.0 mM caffeine (Treatment-2) compared to control, but the 0.5 mM caffeine (Treatment-1), had the highest pre-freeze and post thaw (0hr and 48hr) individual progressive motility percentage compared to other three groups. It was concluded that caffeine at 0.5 mM of concentration showed significant (P< 0.05) improvement in pre-freeze and post thaw semen quality in comparison to concentrations of 1 mM or 1.5 mM; and is sufficient to induce a beneficial effect on cryopreservation of Surti buffalo semen.