Vol. 11, Issue 7 (2022)

Author(s):
Amrinder Singh, Thombre Mahadeo Uttamrao, Tenzin Topgyal, Manmohan Sharma, Gurdev Chand, Manish Sharma and Moni Gupta

Abstract:
Common bean (Phaseolus vulgaris L.) contribute major part of dietary protein for the millions of people across globe, thus making it one of the most legume that can be consumed directly. Study of the genetic diversity of a crop is most important step for understanding genetic variability and its utilization in breeding programmes. Fourteen genomic SSRs were employed for amplifying the genomic regions of 48 common bean (Phaseolus vulgaris L.) lines collected from different regions. The discriminatory power of SSRs was very high owing to their high polymorphic nature. A total of 43 alleles ranging from 2 (BM152) to 4(BM157) were amplified in 48 common bean lines with an average of 3.07 alleles per SSR. Un-weighted neighbour joining cluster analysis was performed and the constructed dendrogram divided 48 common bean lines into 5 major clusters having many sub-clusters. High polymorphism was detected among common bean lines. Most of the common bean lines collected from one region came under one group. The effectiveness of SSR markers was once again validated in this study. Diverse pair of common bean lines can be used for development of improved varieties using breeding programs.