Vol. 6, Issue 11 (2017)

Author(s):
Sunita Arora and Sonam Meena

Abstract:
Plants are important source of secondary metabolites they contain many bioactive constituents with interesting biological activities. Ceropegia bulbosa Roxb. var. bulbosa is an important, endangered herb of Thar Desert of Rajasthan, belongs to Asclepiadaceae (Milk Weed) family. This is a herb of high medicinal value as it is used to cure various diseases like kidney stone, dysentery and deafness. The aim of present investigation was to assess the presences of primary and secondary metabolites including bioactive fractions using certain standard preliminary phytochemical tools and Perkin-Elmer GC-MS with chloroform as a solvent. The mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology and Willey 8 library. Maximum % area is found for Hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester, is present in maximum amount (35.72%) with RT= 24.667 minutes in tuber. Tetracontane is present in maximum amount (41.82%) with RT= 26.255 minutes in stem and 28.24% with RT= 30.583 minutes in leaf of chloroform extract of Ceropegia bulbosa Roxb. var bulbosa. Application of Gas Chromatography Mass Spectrometry (GC-MS) includes environmental analysis, drug detection and identification of unknown samples. Isolation and identification of individual phytochemical constituent and subjecting it to the biological activity will definitely give fruitful results and will open a new area of investigation of pharmacological importance.