Vol. 11, Issue 8 (2022)

Author(s):
Aashitha Joy, SK Manoranjitham, L Karthiba, P Meenakshisundaram and M Suganthy

Abstract:
Papaya, which originated from Central America, is one of the most widely marketed and consumed fruits worldwide. Papaya ringspot virus (PRSV) caused serious havoc in cultivated papaya all over the world. Papaya plants are propagated through seeds and the presence of virus in papaya seeds needs to be ascertained for better management of PRSV. Seeds were collected from PRSV infected and healthy papaya fruits assessed for the presence of PRSV. Seeds with and without sarcotesta did not produce any amplicon in RT-PCR assay using PRSV-specific primers. PRSV infected leaf sample (Positive control) showed an amplicon size of 850 Bp. Hence, the transmission of PRSV through seeds was negligible.