Vol. 11, Issue 8 (2022)

Author(s):
Nimisha V Hirani, HP Gajera, Darshna G Hirpara and RL Bhalara

Abstract:
The plant growth promoting rhizobacteria (PGPR) are naturally occurring soil bacteria which actively colonize plant root and benefit plants by providing growth promotion. The present study reports the analyses of the rhizospheric microbiome of the cumin (Cuminum cyminum L.) interaction with Fusarium wilt. Total 164 isolates were obtained from 10 healthy and Fusarium oxysporum f. sp. Cumini (FoCu) wilt infected rhizosphere under in-vitro condition. After haemolysis test, 60 isolates were found non-pathogenic and further characterised for PGPR and antagonist activity against FoCu. The 45 isolates were positive for IAA production, six isolates were able to ACC (1-aminocyclopropane-1- carboxylic acid) activity, fiveisolates were able solubilise phosphate, eight isolates were able to hydrolysis of chitin and eleven isolates have zinc solubilizing ability under in vitro. The H1-2 and H1-23 isolates were considered as Bacillus subtilus and found with potent antagonist activity. These two bacterial isolates H2 (39.47%) and H23 (52.63%) were suppressed the growth of FoCu. Potent Bacillus isolates derived from microbiome characterisation may be useful as PGPR and antagonist as dual purpose for betterment of cumin cultivation.