Vol. 11, Issue 8 (2022)

Author(s):
Gokulakrishnan M, Suji KK, Priyanka AR, Niranjanadevi J, Balasubramani V, Saraswathi R and Ramalingam J

Abstract:
Simple sequence repeats (SSR) polymorphism in two rice varieties viz., Co51(High yielding, short duration, fine grain variety but susceptible to Brown Plant Hopper) was pyramided with bacterial blight resistance genes xa5, xa13 and Xa21, blast resistance gene Pi54 and sheath blight resistance QTLs qSBR7-1, qSBR11-1 and qSBR11-2 and Ptb33 (BPH resistant line harboring Bph2 and Bph32) was investigated to identify the ready to use SSR markers which can be employed in background selection of marker assisted back cross breeding programme for the introgression of brown plant hopper resistance. A total of 120 genome wide SSR primers were utilized for this parental polymorphism survey equally distributed on all the 12 chromosomes of rice. The results of this survey revealed distinct polymorphism among Co51 and Ptb33 for 51 genome wide SSR markers with a polymorphism information content (PIC) of about 0.5 and polymorphism percentage of about 42.5%. These identified polymorphic SSR markers can be used in crop improvement programs involving Co51 and Ptb33 aiding in marker assisted back ground selection in recombinants there by developing a desirable cultivar resistant to multiple stress.