Vol. 11, Issue 8 (2022)

Author(s):
Jyoti Singh, RS Sengar, Mukesh Kumar, Vaishali, Manoj Kumar Yadav and Pooranchand

Abstract:
Banana is one of the most crucial fruit crop and food source for the million of people in developing countries. Now a days, efficient technique of micropropagation has gained popularity because of the uniformity, superiority, disease free and high yielding. Mainly the in vitro micropropagation is affected by the growth of contaminants which can cause negative effect on the in vitro culture establishment that can be remove by the utilization of different effective sterilization agents to remove the damaging negative effect. Keeping in this view, this study was conducted to standardized the effect of Sterilization procedure for grand naine banana genotype. The healthy explants of banana (Musa spp.) were collected and treated with different combination of sterilization agents at different concentrations i.e., Bavistin TM (1%), HgCl₂ (0.01-0.5%), NaOCl (5%) and EtOH (70%). After the proper sterilization step, the treated explants were directly inoculated on semi-solid MS media supplemented with 5 mg/LBAP + 1 mg/L IAA. The findings we recorded under the current study showed the significant differences in their activity. The optimum % culture survival and contamination with reduced necrosis was found when the Banana explants were treated with the combination of 0.1% Bavistin for 30 seconds, followed by 0.1% HgCl₂ for 5min, 5% NaOCl for 5min and 70% EtOH for 30 sec. This sterilization protocol will be contributive in efficient surface sterilization for in-vitro propagation of Grand naine genotype of Banana. Results indicated that a treatment combination no. S16 [Bavistin (1.0%) + Mercuric chloride (0.1%) + Sodium hypochlorite (5%) + Ethanol (70%)] gave the highest percentage of aseptic culture establishment in- vitro condition.