Vol. 11, Issue 8 (2022)

Author(s):
Pavithra H, Sakthi AR, Raveendran M, Rajasree V and Sudha M

Abstract:
Tomato (Solanum lycopersicum L.) is an important vegetable crop cultivated throughout India. A good in vitro plant regeneration system for mass propagation of high-quality and disease-resistant tomato via genetic engineering is required for further improvement of the commercially important cultivars. In vitro plant regeneration was assessed in tomato (Solanum lycopersicum.cv. PKM1) using cotyledon and hypocotyl explants from seven to eight days old aseptically grown seedlings. Shoot initiation was achieved by culturing cotyledon and hypocotyl explants on Murashige and Skoog (MS) medium supplemented with different concentrations of kanamycin and hygromycin. The effects of two antibiotics namely hygromycin and kanamycin, on the shoot regeneration of co-cultivated explants of PKM1variety cultured on MS medium with phytohormones, were studied. The ability of tomato cotyledon and hypocotyl explants to regenerate into entire plants via direct organogenesis was tested and drying of explants was observed after two weeks of subculture in all concentrations under both antibiotics. Shoot initiation was started after two weeks of subculture in kanamycin ata concentration of100mg/land three weeks of subculture in hygromycin at 10mg/l.