Vol. 11, Issue 8 (2022)

Author(s):
Rothe AS, Narute TK, Raghuwanshi KS and Kolase SV

Abstract:
The Xanthomonas axonopodis pv. punicae causing bacterial blight disease of pomegranate was isolated from diseased leaves and fruits samples collected from Farmers field. Cultural characteristics viz. colour of colony, growth on the media, growth appearance, elevations and texture and consistency and biochemical tests helps to identification of bacteria. Different cultural media were Use to study the cultural characteristics viz. Nutrient Agar (NA), Nutrient Sucrose Agar (NSA), Glucose Yeast Chalk Agar (GYCA) and Yeast Extract Dextrose Calcium Carbonate Agar (YDCA). Gram staining test, potassium hydroxide test, starch hydrolysis and catalase test were performed to characterized the X. axonopodis pv. punicae. The results of cultural, morphological and biochemical tests were confirmed the X. axonopodis pv. punicae a Gram negative bacterium.