Vol. 11, Issue 9 (2022)

Author(s):
AC Patil, KT Apet and DS Kadam

Abstract:
Ginger crop is one of the most important spice crops cultivated in India. Which suffers from several diseases, among which rhizome rot complex caused by P. aphanidermatum, F. oxysporum f. sp. zingiberi, S. rolfsii, R. solanacearum and M. arenaria is one of the serious diseases observed regularly in ginger growing areas. As major pathogen for rhizome rot is P. aphanidermatum, on that basis molecular studies were conducted. Molecular variability of eight isolates of P. aphanidermatum was studied applying STR PCR, using 14 primers viz. P18CCA1-41, P18TGA2-9, P18TTC3-29, P18CA2-23, P18GAA1-71, P18TCC3-25, P18GAA1-72, P18GAA3-34, P18GT4-20, P18CAT1-74, P18TTG1-79, P18ACA2-81, P18AC2-18, and P18TTC1-42. The dendrogram analysis revealed phylogenetic relationship of pathogen in two clusters. In the Cluster I, four isolates of P. aphanidermatum viz., Iso-2, Iso-29, Iso-7 and Iso-36 found. Cluster II comprised of four isolates viz., Iso-14, Iso-17, Iso-32 Iso-33.