Vol. 11, Issue 9 (2022)

Author(s):
Athira VB, M Balakrishnan, S Karthikeyan, S Marimuthu and S Ganapathy

Abstract:
In this study, lipase was extracted from Carica papaya latex and its enzymatic activity was evaluated at different time (6-24 h), temperature (35-50), and enzyme concentration (5-25%). The lipase was extracted from papaya latex by the conventional extraction method. The papaya latex was collected and further processed by centrifugation, washing, and lyophilization to get agglomerated Carica papaya lipase powder. The enzyme activity of extracted lipase was evaluated with tween 20 and coconut oil as substrates. The enzyme activity was maximum at the time of 12 h of incubation period, temperature of 45 °C, and with an enzyme concentration of 20% (w/w of substrate). This study revealed that Extracted Carica papaya lipase exhibited a powerful lipase activity for catalyzing hydrolysis (esterification and interesterification type reactions). The maximum enzyme activity for both substrates was seen after 12 hours of incubation. The maximum enzyme activity of lipase was 66.87 U/mL and 63.45 U/mL respectively when tween 20 and coconut were used as the substrates for 12 h time period. For both substrates, 45 °C temperature resulted in the highest lipase activity. When using tween 20 as the substrate, the lipase activity of 37.87 U/mL was attained at a temperature of 45 °C. When coconut oil served as the substrate, the enzyme activity peaked at 35.71U/mL at the same temperature. The enzyme concentration with 20% substrate for both substrates produced the highest level of lipase activity. Using tween 20 as the substrate, the enzyme had a lipase activity of 33.54 U/mL at a 20% concentration. At the same enzyme concentration, the lipase activity with coconut oil as the substrate was 30.30 U/mL. The extracted Carica papaya lipase can be used for the synthesis of monolaurin from coconut oil by enzymatic glycerolysis.