Vol. 11, Issue 9 (2022)

Five varities were tested to detect the seed borne mycoflora by standard blotter paper method, pre-treatment blotter method and agar plate method. It was revealed that six fungal species associated viz., Fusarium oxysporum, Aspergillus niger, Aspergillus flavus, Alternaria alternata, Curvularia lunata and Cladosporium spp. Fusarium oxysporum found major fungus associate with all sesamum varieties.

Three fungicides and two botanicals were tested against Fusarium oxysporum under in vitro condition. Among the three fungicides carbendazim + mancozeb and hexaconazole completely inhibited the mycelial growth (100%) followed by tebuconazole (88.82%). Among botanicals neem leaf extract inhibited maximum mycelial growth (77.34%) Allium sativum (72.20%). Four bioagents viz., Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescens and Bacillus subtilis were tested against Fusarium oxysporum by dual culture method. Maximum mycelium growth inhibition observed in Trichoderma harzianum (75.85%) followed by Trichoderma viride (73.68%) and Bacillus subtilis (72.44%).