Cloning and in silico analysis of the upstream region of an endodermis specific promoter in rice
Author(s):
RY Manoj, KK Kumar, E Kokiladevi, S Varanavasiappan, D Sudhakar and L Arul
Abstract:
SCARECROW (SCR) is a plant-specific transcription regulator that is well-known for its role in stem-cell regeneration in plant roots. SCR is expressed in the initial cells of the cortex/endoderm and the endodermal cell lineage. Tissue specific expression of SCR is regulated at the transcriptional level. In this study a 1.29 kbp upstream region of the SCARECROW gene was demarcated and analyzed for the Cis-regulatory elements and transcription start site (TSS). Through PCR the 1.29 kbp was cloned using specific primers from the rice genotype cv. Mundan, a salt tolerant landrace. Analysis of potential cis-elements using Plant CARE database deduced 24 cis-regulatory elements and one TSS.
How to cite this article:
RY Manoj, KK Kumar, E Kokiladevi, S Varanavasiappan, D Sudhakar, L Arul. Cloning and in silico analysis of the upstream region of an endodermis specific promoter in rice. Pharma Innovation 2022;11(9):2063-2067.