Vol. 11, Issue 9 (2022)

Author(s):
Prateek Ranjan Behera, MK Mishra, Sandeep Kumar and AK Senapati

Abstract:
Yellow mosaic virus disease (YMV) infecting mungbean is one of the most important cause responsible for vast scale yield loss in India and across the globe. In the present study, the transmissibility of YMV through sap and seed were examined. Mechanical transmission of the YMV either using 2% celite with carborundum powder or β mercataethanol along with virus extract in three healthy mungbean varieties was found to be unsuccessful as there was no symptom development 20-30 days after incoculation and the leaf samples tested negative in PCR amplification. There was 16.7 % reduced germination in the mungbean seeds collected from YMV infected plants. Although the grow-out test of seeds collected from the YMV infected mungbean plants showed no development of symptoms, there was detection of YMV in 20% plants selected arbitrarily 30 days after sowing in PCR test using the coat protein genome specific of the legume infecting begomoviruses. PCR amplification of different parts of germinated seeds showed the presence of the virus in cotyledon, cotyledonary leaves, epicotyl except seed coat.