Vol. 11, Issue 9 (2022)

Author(s):
Prateek Ranjan Behera, MK Mishra, Sandeep Kumar and AK Senapati

Abstract:
Mungbean (Vigna radiata L.) is a short duration pulse crop cultivated under varying environmental conditions throughout the southeast Asia and particularly in India. Yellow mosaic disease caused by yellow mosaic virus transmitted by whitefly (Bemisia tabaci) is an important limiting factor for the production and productivity of green gram. In the present study, different virus-vector relationships have been studied. A single whitefly could transmit 30% yellow mosaic disease in 10 days old greengram (Variety. LGG-460) after 24 hr Acquisition access period. The rate of transmissibility increased by increasing the number of viruliferous whiteflies with minimum of 13 numbers attaining 100% transmission. Minimum 15 hr acquisition access period (AAP) was required for 100% transmission of YMV in greengram plants. Also, minimum 15 hr latent period and inoculation Access Period (IAP) were required for 100% transmission of yellow mosaic virus in greengram by minimum of 13-15 number of viruliferous whiteflies. This study also revealed that maximum 100% infection in 10 days old plants and declined afterwards upto 40% transmission in 21 days old greengram plants. The PCR amplification of symptomatic leaf samples from the inoculated plants also indicated ~ 450 bp band of genomic region of yellow mosaic viruses of begomovirus genus.