Toll Free Helpline (India): 1800 1234 070

Rest of World: +91-9810852116

Free Publication Certificate

Vol. 11, Issue 7 (2022)

Detection and Isolation of seed mycoflora of lablab bean, horse gram and cowpea and control of major seed mycoflora

Author(s):
YK Nirgude, RR Rathod, UR Phondekar, Rohini Gaonkar and NV Chaure
Abstract:
Seed is primary most important input in Agriculture. Healthy seeds produce healthy crops and good yields. Most of the plants grown by seeds so it is more important to protect the seeds from pathogens. Seed quality depends on presence or absence of seed borne mycoflora. Lablab bean Horse gram and Cowpea are important legumes in konkan region. They are rich in nutrients content and medicinally important. The seeds are infected by many seed borne fungi which resulted in poor germination, plant growth vigor, seedling mortality and storability. To detect and isolate the seed borne mycoflora various seed health testing methods were attempted i.e. Blotter paper method, Agar plate method and PDA method. All the methods were found effective to detect the seed borne mycoflora. The in vitro efficacy of fungicides against major seed borne fungi were tested. All tested fungicides viz, Carbendazim 50% WP @ 0.2% (100%), Benomyl 50% WP @ 0.2% (85.18%), Thiophanate methyl 70% WP @ 0.1% (85.18%), Carboxin 75% WP @ 0.25% (68.88%), Mancozeb 75% WP @ 0.25% (67.40%), Thiram 75% WP @ 00.2 (66.66%) and Captan 75% WP @ 0.30% (61.11%) were found effective against mycelial growth inhibition of Alternaria alternata and (Carbendazim 50% WP @ 0.2%, (100%), Benomyl 50% WP @ 0.2% (88.88%), followed by Thiophanate methyl 70% WP @ 0.1% (87.03%), Carboxin 75% WP @ 0.25% (83.33%), Captan 75% WP @ 0.30% (62.22%), Thiram 75% WP @ 0.2% (60.37%) and Mancozeb 75% WP @ 0.25% (58.88%) were found effective mycelial growth inhibition of Fusarium oxysporum.
Pages: 3768-3774  |  221 Views  134 Downloads


The Pharma Innovation Journal
How to cite this article:
YK Nirgude, RR Rathod, UR Phondekar, Rohini Gaonkar, NV Chaure. Detection and Isolation of seed mycoflora of lablab bean, horse gram and cowpea and control of major seed mycoflora. Pharma Innovation 2022;11(7):3768-3774.

Call for book chapter