Vol. 11, Issue 10 (2022)

Author(s):
Abdul Sadam, Madhuri Patel, Sanjay Kumawat, W Ramdas Singh, Anshuk Sharma, Aneesha VA, Madhu CL and Dinesh Kumar

Abstract:
Wound healing is an essential physiological process consisting of the collaboration of many cell strains and their products but it gets impaired and delayed in many pathological conditions, like diabetes mellitus. The aim of present study was to assess the healing potential and to calculate the EC₅₀ of Hemin in diabetic wound model. Diabetes was induced in rats by injecting streptozotocin, and the 400 mm2 wounds were created on the dorsal thoracic region. Ointments of different Hemin concentrations (0.05% Hemin, 0.1% Hemin, 0.5% Hemin and 2.5% Hemin) were applied topically on the wound, twice daily, for 7 days. In control group, only ointment base was applied. Wound area was photographed and measured by tracing its contour using a transparent sheet on days 0, 3, 5 and 7 post-wounding. Rats were sacrificed on day 7 post-wounding, and the granulation tissues were collected for assessing hydroxyproline and glucosamine levels. 7^th day wound contraction data was used to calculate EC₅₀ by inverse linear regression analysis. Grossly there was considerable change in the wound size in the treatment groups compared to control group particularly from 5th day onwards. Hemin-treated groups, specifically 0.5% and 2.5%, showed higher wound contraction on 5th day onwards as compared to control group, and also there was increase in the levels of hydroxyproline and glucosamine in hemin-treated groups compared to control group. Further, in the present study, the experimental EC₅₀ of Hemin is 0.96%. Based on our findings, it can be concluded that, in the present study, topical application of Hemin seemed to have played crucial role in improved wound contraction as well as better maturation of the healing tissue.