Vol. 11, Issue 10 (2022)

Author(s):
Mangesh Raut, Sunita J Waghmare and DP Deshmukh

Abstract:
Fifteen Azotobacter and five phosphate solubilizing fungal (PSF) were isolated from chilli rhizosphere of Kolhapur District and characterized by using both morphological and biochemical tests for cell shape, colony size, gram reaction and catalase activity were identified as Azotobactor. All isolates of Azotobactor were gram negative. Eleven Azotobactor isolates were cocci in shape and three were bacillus. Cell arrangement was observed as scattered in chain in 8 isolates and 7 isolates were singly scattered. Thirteen isolates showed positive results for the methyl red test and catalase test except two isolate. The results of starch hydrolase test were showed positive by fourteen isolates except one isolate whereas all isolates indicated positive results for the gelatine hydrolysis test, gas production test and H2S production test. Fourteen isolates showed positive results for oxidase test except one isolate. Five phosphate solubilizing fungal isolates were showed white and black colonies producing brown, black and green spores with smooth and rough colony surface. Mycelium hyaline and septate which producing conidia with globuse black, brown brush like phialides with green conidia. Finally from above characteristics five isolates, three Aspergillus sp. and two Penicillium sp. were identified as phosphate solubilizing fungal isolates.