Vol. 11, Issue 10 (2022)

“A protocol for in vitro regeneration of mature plants of Philippine lime and yellofw citrus fruit using plant part as explant such as seed has been developed for multiplication from seed concentration of BAP, KIN and NAA were used from them. Highest seed germination in terms of the total number of Explant observed in half concentration of MS media containing BAP + NAA + KIN. The establishment of sterile in vitro regeneration of Philippine lime and yellow citrus fruit is a major challenge because of contamination. The use of multiple concentrations of alcohol and sodium hypochlorite along with tween-20 was considered the best treatment for the reduction of contaminants”.