Vol. 11, Issue 10 (2022)

Author(s):
Ramesh Kankanawadi, Basavaraj Awati, Ambadas and Shakuntala Kage

Abstract:
The present study was designed to study the cultural and morphological characterization of the Salmonella spp. from pigs. A total of 175 feacal samples collected from different pig farms which are located in Bidar, Hassan and Tumakuru districts of Karnataka out of which 24feacal samples were found to be positive for Salmonella spp. Based on the morphological and cultural characters. Out of 175 suspected diarrhoeic cases total number of isolates were 24 with 13.71 percent prevalence rate. The colony characters showed on different selective media are Hichrome Salmonella agar showed transparent colonies with black centres due to production of H₂S, pink colonies with black centre on XLD agar due to production of H₂S, colourless or red to pinkish white colonies often surrounded by pink or red zone on BGA agar because the bacterium does not ferment lactose or sucrose and Non-lactose fermenter Salmonella spp. colonies appear colourless and transparent on MacConkey agar plates and typically did not alter appearance of the medium and colourless with transparent colonies on nutrient agar. On Gram’s staining, the morphologically isolated bacteria was small rod shaped, Gram negative, single or paired in arrangement.