Vol. 11, Issue 10 (2022)

The advantage of technology has provided better options to combat today's hurdles. In the era of modern agriculture, the unmanageable use of pesticides and fungicides are causing severe health problems. On the other side, phytopathogens are developing more résistance against traditional chemical measures. Thus, need of time to alternative cost-effective and eco-friendly technology for the solution to this modern problem. Furthermore, nanotechnology can be utilised to resolve these challenges. The green synthesis of nanoparticles has novel surface-dependent properties like catalytic and antimicrobial activity. Therefore, in present study the evaluation of silver NPs via the biogenic method of synthesis from mango leaf was carried out. These silver NPs were used to check the inhibitory effect against anthracnose disease in in-vitro conditions. The synthesized silver NPs exhibited 432 nm Surface Plasmon Resonance with calculated band gap energy of 2.87 eV. DLS analysis found an average particle size of 64.87 nm and the zeta potential peak at -14.0 mV. The TEM analysis revealed almost spherical shapes of synthesized silver NPs with an average size of 33.90 nm. In the present study aqueous biogenic silver NPs exhibited potent anti-fungal activity against C. gloeosporioides growth with inhibiting areas of 4.74±0.13 mm, 6.04±0.19 mm, 8.29±0.06 mm and 9.12±0.30 mm at 50, 100, 150 and 200 ppm concentrations, respectively. This was found very significant and advisable against positive control with higher concentrations of carbendazim (1000 ppm). Thus nanotechnology could be a better alternative approach to control anthracnose disease in the field and storage condition of mango.