Vol. 11, Issue 10 (2022)

Author(s):
KR Sreya, R Uma, KK Jayavardhanan and Preethy John

Abstract:
Nanoparticles have a wide range of applications in the field of medical science and can be synthesized using a variety of physical, chemical and biological methods. The present study focuses on the green synthesis of silver nanoparticles using Oxalis corniculata leaf extract and evaluation of their antibacterial activity. The extract which revealed the presence of alkaloids, glycosides, flavonoids and diterpenes on phytochemical analysis was used for the synthesis of nanoparticles using three different concentrations of silver nitrate i.e., 1 mM, 3 mM and 5 mM. Colour change of the solution from yellow to dark brown due to surface Plasmon resonance confirmed the formation of nanoparticles which were characterized by UV-visible spectroscopy, Fourier transform-infrared spectroscopy, X-ray diffraction and field emission scanning electron microscopy. The synthesized nanoparticles were found to possess excellent antibacterial activity against Gram positive Staphylococcus aureus and Gram negative Escherichia Coli. The nanoparticles formed of 1 mM silver nitrate were found to be more effective against Gram negative bacteria while those made of 3 mM silver nitrate were more active against Gram positive bacteria. It could be observed that the minimum inhibitory concentration and minimum bactericidal concentration of the nanoparticles were 20µg/mL against both E Coli and S Aureus.