Vol. 11, Issue 10 (2022)

The Observation of the present investigation was conducted for screening of forty five inbreeds under normal moisture (30 kpa) and under controlled moisture stress (50 kpa) condition at rainout shelter (rabi 2019-20) in crop season–I and after selection of 12 inbreeds among forty five, single cross hybrids were obtained by crossing 8 lines and 4 tester L X T fashion and thirty two hybrids were developed whereas in crop season-II Screening of forty eight genotypes under normal moisture condition (30 kpa) experiment in W section of research farm (Kharif 2019-20) and controlled moisture condition under rainout shelter (50 kpa) experiment was conducted.

The development of drought tolerant and diverse varieties in maize may improve the performance of the crop even under water stressed conditions, and hence increase yield to meet the food requirements of the people of Jharkhand Genetic diversity is of prime importance for the successful adaptation to certain agro-climatic conditions and improvement of any crop species. For the purpose of this study the simple sequence repeat (SSR) markers (microsatellites) were used to screen for diversity and drought. In the present study forty five inbred lines of maize and twelve maize hybrids were evaluated through fourteen polymorphic SSR markers. Each detected band was considered to be an allele. Variations in alleles were recorded to generate the molecular data viz., number of alleles per locus (k), observed Polymorphic Information Content (PIC). A total number of 705 alleles were generated by fourteen polymorphic SSR markers among the five inbred lines of maize. The number of alleles ranged from 1-4 with an average of 2.85 alleles per polymorphic primer pair. The highest number of alleles were observed in zmDi 19-1 & gras20 (four alleles) and result showed moderately PIC value. Cluster analysis was carried out based on similarity index data derived from the SSR markers which grouped forty five inbreeds and twelve hybrids into two major clusters, cluster A and cluster B. The jaccard’s similarity coefficient ranged from 0.52 to 0.95. Further this 2 main cluster classified for six subcluster. Every genotypes name are mentioned that comes under different cluster and subcluster. Further Cluster A showed four subcluster and Cluster B showed two subcluster. Drought stress leads to reduction of genetic variance components and heritability, the results of this study will be helpful in giving basic information about indirect selection of traits through associated markers and hybrids are drought tolerant and diversified can be further used for the experimental studies.