Vol. 11, Issue 10 (2022)

Author(s):
Pansuriya Namrata B, Hardik S Lad, HR Ramani and Rajkumar BK

Abstract:
The experiment entitled “screening the cotton (Gossypium hirsutum L.) Genotypes for drought tolerance” was conducted at Department of Biochemistry, Main Cotton Research Station, Navsari Agricultural University, Surat. Twelve genotypes were selected for screening in drought tolerance. Seeds were grown in pots filled with sand culture for 15 days. The seedling was subjected to control and 10 % PEG-6000 treatment for 48 hour and control was taken without PEG treatment. Samples were analyzed for relative water content, membrane stability index, proline, chlorophyll stability index and molecular diversity using molecular markers. Two genotypes GSHV-172 and GISV-272 showed highest relative water content (70.36 and 72.31 %) in PEG treatment and (95.83 and 95.71 %) respectively in control while membrane stability index was found (73.03 and 66.49 %) in GSHV-172 and GISV-272 respectively in PEG treated samples. Proline contend was found higher in same genotype. Two genotypes GSHV-172 and GISV-272 showed highest Chlorophyll stability index compared to other genotypes. So based on biochemical parameters GSHV-172 and GISV-272 found drought tolerant genotype, rest of genotypes were moderately tolerance to drought susceptible. Total twelve RAPD were amplified to generate the 86 fragments and obtained 71 polymorphic bends. The percent polymorphism obtained for RAPD primers were ranked from 25 % to 100 %. The cluster analysis showed the highest similarity 94 % was observed between GISV-272 and GSHV-172 while, the lowest similarity 77 % was observed between Surat dwarf and GSHV-01/13387.