Vol. 11, Issue 11 (2022)

Spinach (Spinacia oleracea L.), is an annual, herbaceous, nutritionally dense leafy vegetable belonging to family Amaranthaceae. Spinach is attacked by various fungal diseases like damping off and leaf spot. Among these, leaf spot caused by Cercospora beticola is one of the limiting factors in commercial cultivation of palak. Fructification study was carried on Richards Agar media impregnated with various vitamins and the growth and sporulation was recorded significantly superior with the incorporation of vitamin combination of thiamine + pyridoxine + choline, followed by thiamine + pyridoxine. Minimum growth and sporulation was observed with the addition of pyridoxine alone with no sporulation when devoid of any vitamin. The results clearly indicate that the vitamins play significant role in the fungal reproduction.