Vol. 7, Issue 1 (2018)

Author(s):
Priyanka Arya, RS Chauhan and Iqra Nazir

Abstract:
The present study was designed to evaluate haematological and biochemical effect of commercial nutraceutical Stimulin on the fingerlings of Indian major carp Labeo rohita. Therefore, the fingerlings with average weight of 7.203 ± 0.24 g were distributed randomly into four treatment groups T1, T2, T3 and T4. Experimental diets were prepared by mixing rice bran, deoiled mustard oil cake, deoiled soybean cake and vitamin mineral mixture. The nutraceutical Stimulin was incorporated into diet D2 @ 0.5%, D3 @ 0.75% andD4 @ 1.00%. In control diet D1_, Stimulin was not incorporated. T1 group fishes were fed with D1 diet, T2 with D2, T3 with D3@ 5% body weight per day for 90 days. The water quality parameters were regularly monitored. Fingerlings fed with diet D4 in T4 group achieved higher value of total leukocyte counts (33.646 × 10³/µl), total erythrocyte counts (3.26× 10⁶/µl), haemolglobin (9.9 g %), total serum protein (8.8 g/dl), albumin (1.445 g/dl) and globulin (7.358 g/dl) as compared to control and all other treatments. Haematological examination was carried out to estimate the effect of experimental diet on the fish to evaluate the growth, stress resistance and specific and non specific immunity of the fish. The obtained results indicated that T4 was the best treatment which realized significant (P<0.05) increase in all haematological and biochemical parameters. The results indicated that Stimulin has very good immunostimulating and does not have any adverse effect in health of raising Labeo rohita in culture system.