Vol. 11, Issue 11 (2022)

Studies were carried to evaluate the effect of different growth media, temperature, pH on mass multiplication of probiotic bacterial biocontrol agent and different carrier based materials for bio formulation and to know the shelf life under different storage temperature conditions. Superior isolate Lactiplantibacillus plantarum NRB14 showing plant growth promotion and antifungal activity against chilli diseases under in-vitro and in-vivo condition were selected and evaluated to standardize media and carrier materials for bio-formulation. Among different growth media compositions highest biomass mass production was noticed in MRS broth with population count of 8.21 (log 10 cfu/ml) followed by molasses+ yeast extract with cell biomass of 8.10 (log10 cfu/ml) and lowest cell biomass was noticed in sucrose+ yeast extract 7.72(log10 cfu/ml) 48 h after incubation. Ideal temperature of 30-35 °C and pH of 6 to 7 was congenial for better biomass production. Amongst different carrier materials used, liquid based bio formulation amended with glycerol showed highest population density of (7.67 log10 cfu/ml) followed by talc based formulation (7.37 log 10 cfu/g) with shelf life period of 210 days at 28 °C storage temperature condition. Finally conclude that MRS broth and molasses + yeast extract broth showed good growth of bacteria for mass multiplication of biocontrol agent and liquid and talc based formulation showed shelf life period of 210 days at room temperature storage condition.