Vol. 11, Issue 11 (2022)

Bean common mosaic virus (BCMV) associated with field bean in GKVK (BCMV-Fb-GKVK) isolate was mechanically inoculated to different host plants. The host range studies were carried out to determine the biological relationship of BCMV-Fb-GKVK isolate with other host plants through mechanical sap inoculation method. Out of 22 host plants tested, BCMV was easily transferred to 11 different host plants. The incubation period taken for symptom expression was varied within the plant species. Soybean taken longer incubation period of 10-15 days for expression of symptoms like mosaic and veinal chlorosis. Whereas, lesser incubation period was noticed in Chenapodium (1-2 days) and tobacco (6-7 days). All plants were subjected to Double antibody sandwich- Enzyme linked immunosorbent assay (DAS-ELISA) to confirm the presence of BCMV. The results showed that, the host plants whichever exhibited different symptoms upon mechanical sap inoculation have reacted positively with the antibody specific to BCMV and produced bright yellow color thus, confirmed the presence of BCMV.