Abstract:Five forest species viz. Bamboo, Teak, Eucalyptus, Accacia and Neem were investigated for arbuscular mycorrhizal association from three different locations viz. Nagpur, Ramtek and Umred region to of Maharashtra. The results indicated that AM spore isolated by using wet-sieving and decanting method. All forest species were infected by arbuscular mycorrhiza as found by Trypan blue staining procedure. Based on correlation analysis, physico-chemical properties like Soil pH, EC, OM% and available nutrients play an important role in mycorrhizal population in forest species. One way ANOVA analysis was done to know the effects of physico-chemical properties on mycorrizal population and the conclusion was drawn that soil physico-chemical properties like Soil pH, EC, OM% and available nutrients showed significant difference in forest species like Bamboo, Teak, Eucalyptus, Accacia and Neem.